Product Description: IgG-Cutter^TM is a recombinant IgG endoproteinase (Mac/IdeS) from Streptococcus pyogenes. It can specifically cleaves peptide bonds in the hinge region of IgG to form Fc and F(ab’)2 fragments. IgG-Cutter^TM cleaves all subclasses of human, rabbit, sheep, and monkey IgG with a high efficiency. In addition, IgG-Cutter^TM can cleave IgG2a and IgG3 of mouse IgG.

Product Source: IgG-Cutter^TM was produced in E. Coli cells transformed with Mac/IdeS gene. This product is sterile and does not contain any components of animal origin.

Reaction Conditions: 20 mM Tris buffer pH 8.0; incubate at 37°C for at least 30 minutes. Cleavage reaction can also be carried out at room temperature using longer time.

Unit Definition: One unit is defined as the amount of enzyme required to digest >95% of 1 ug of human IgG in 30 minutes at 37°C.

Form: Sterile liquid formulation. 20 mM Tris buffer pH 8.0, 50% Glycerol, and 5 mM b-Mercaptoethanol.

Usage: FOR LABORATORY RESEARCH USE ONLY.

Compatible buffers

Phophate buffer saline(PBS) pH 6.0-8.0

Tris buffer pH 7.0-8.0

MES buffer pH 5.5-6.5

HEPES buffer 7.0-8.0

Ammonium bicarbonate buffer 6.0-7.0

Sodium acetate buffer 6.0

Background: IgG-Cutter^TM is recombinant IgG endoproteinase (Mac/IdeS) from Streptococcus pyogenes. The complete genome sequencing of M1 group A Streptococcus (GAS) was done in 2001 (Ferretti 2001). To our knowledge, Mac/IdeS was first identified in group A Streptococcus (GAS) infected mice and human sera as a homologous protein to Human Mac-1 at NIH (Lei 2000). Mac/IdeS was later found to promote inhibition of opsonophagocytosis, enhancement of pathogen survival, and establishment of infection and dissemination (Lei 2001). Several groups later characterized Mac/IdeS’s IgG endoproteinase activity, and found it to cleave peptide bonds in the hinge region of IgG (von Pawel-Rammingen 2002, Lei 2002). 

References:

1. Ferretti JJ, McShan WM, Ajdic D. et al (2001) Proc.Natl. Acad. Sci. USA, 98, 4658-4663.

2. Lei, B., Mackie, S., Lukomski,  S., Musser, M. M. (2000) infect. Immun. 68, 6807-6818.

3. Lei B, Deleo FR, Hoe NP. Et al (2001) Nat. Med. 7, 1298-1305

4. 2. von Pawel-Rammingen, U., Johansson, B. P. & Bjorck, L. (2002) EMBO J. 21,      1607-1615.

5. Lei, B., DeLeo, F. R., Reid, S. D., Voyich, J. M., Magoun, L., Liu, M., Braughton, K. R., Ricklefs, S., Hoe, N. P., Cole, R. L., et al. (2002) infect. Immun. 70,  6880-6890.