Type

Mouse Antibody Monoclonal

Introduction

A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.

Clone

NYRFLAG

Immunogen

Flag-conjugated proteins

Ig Subclass

Mouse IgG1/kappa

Purification Method

Protein A column.

Usage

FOR LABORATORY RESEARCH USE ONLY.

Applications

WB, IP, IF